Phosphorus-31 nuclear magnetic resonance studies of phosphorylated proteins
M Brauer, BD Sykes - Methods in Enzymology, 1984 - Elsevier
Publisher Summary This chapter focuses on the characterization of phosphorylated target
protein by phosphorus-31 nuclear magnetic resonance (31P NMR). It describes the
preparation of a sample of phosphorylated protein for analysis by 31P NMR. 31P NMR can
be used to assign the type of amino acid residue the phosphate is covalently attached to,
such as the β-hydroxyl of a serine or threonine residue, or the N-1 or N-3 nitrogen of the
imidazole ring of a histidine. 31P NMR can provide a range of information about the nature …
protein by phosphorus-31 nuclear magnetic resonance (31P NMR). It describes the
preparation of a sample of phosphorylated protein for analysis by 31P NMR. 31P NMR can
be used to assign the type of amino acid residue the phosphate is covalently attached to,
such as the β-hydroxyl of a serine or threonine residue, or the N-1 or N-3 nitrogen of the
imidazole ring of a histidine. 31P NMR can provide a range of information about the nature …