[HTML][HTML] An experimentally based computer search identifies unstructured membrane-binding sites in proteins: application to class I myosins, PAKS, and CARMIL
H Brzeska, J Guag, K Remmert, S Chacko… - Journal of Biological …, 2010 - ASBMB
Programs exist for searching protein sequences for potential membrane-penetrating
segments (hydrophobic regions) and for lipid-binding sites with highly defined tertiary
structures, such as PH, FERM, C2, ENTH, and other domains. However, a rapidly growing
number of membrane-associated proteins (including cytoskeletal proteins, kinases, GTP-
binding proteins, and their effectors) bind lipids through less structured regions. Here, we
describe the development and testing of a simple computer search program that identifies …
segments (hydrophobic regions) and for lipid-binding sites with highly defined tertiary
structures, such as PH, FERM, C2, ENTH, and other domains. However, a rapidly growing
number of membrane-associated proteins (including cytoskeletal proteins, kinases, GTP-
binding proteins, and their effectors) bind lipids through less structured regions. Here, we
describe the development and testing of a simple computer search program that identifies …