[HTML][HTML] LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells
G Maertens, P Cherepanov, W Pluymers… - Journal of Biological …, 2003 - ASBMB
We have reported that human immunodeficiency virus type 1 (HIV-1) integrase (IN) forms a
specific nuclear complex with human lens epithelium-derived growth factor/transcription co-
activator p75 (LEDGF/p75) protein. We now studied the IN-LEDGF/p75 interaction and
nuclear import of IN in living cells using fusions of IN and LEDGF/p75 with enhanced green
fluorescent protein and far-red fluorescent protein HcRed1. We show that both the N-
terminal zinc binding domain and the central core domains of IN are involved in the …
specific nuclear complex with human lens epithelium-derived growth factor/transcription co-
activator p75 (LEDGF/p75) protein. We now studied the IN-LEDGF/p75 interaction and
nuclear import of IN in living cells using fusions of IN and LEDGF/p75 with enhanced green
fluorescent protein and far-red fluorescent protein HcRed1. We show that both the N-
terminal zinc binding domain and the central core domains of IN are involved in the …