A subtractive PCR-based cDNA library made from fetal thymic stromal cells
MG Kim, C Chen, FA Flomerfelt, RN Germain… - Journal of …, 1998 - Elsevier
We describe our initial approach to clone and characterize genes expressed preferentially in
thymic stromal cells, in an attempt to generate molecular reagents to study the role of these
cells in thymopoiesis and thymic function. Thymic stromal cells were prepared from fetal
thymic organ cultures by treating them with 2-deoxyguanosine and depleting the remaining
hematopoietic cells with anti-CD45 antibody. A cDNA library was then prepared after
subtraction and amplification by PCR. The cloned inserts were sequenced and compared for …
thymic stromal cells, in an attempt to generate molecular reagents to study the role of these
cells in thymopoiesis and thymic function. Thymic stromal cells were prepared from fetal
thymic organ cultures by treating them with 2-deoxyguanosine and depleting the remaining
hematopoietic cells with anti-CD45 antibody. A cDNA library was then prepared after
subtraction and amplification by PCR. The cloned inserts were sequenced and compared for …