[HTML][HTML] Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg
D Baba, S Kashiwabara, A Honda, K Yamagata… - Journal of Biological …, 2002 - ASBMB
The function of glycosylphosphatidylinositol-anchored sperm hyaluronidase PH-20 in
fertilization has long been believed to enable acrosome-intact sperm to pass through the
layer of cumulus cells and reach the egg zona pellucida. In this study, we have produced
mice carrying a null mutation in the PH-20 gene using homologous recombination. Despite
the absence of sperm PH-20, the mutant male mice were still fertile. In vitro fertilization
assays showed that mouse sperm lacking PH-20 possess a reduced ability to disperse …
fertilization has long been believed to enable acrosome-intact sperm to pass through the
layer of cumulus cells and reach the egg zona pellucida. In this study, we have produced
mice carrying a null mutation in the PH-20 gene using homologous recombination. Despite
the absence of sperm PH-20, the mutant male mice were still fertile. In vitro fertilization
assays showed that mouse sperm lacking PH-20 possess a reduced ability to disperse …
